RESUMO
A new phenolate-thiazole derivative (L) has been synthesized and structurally characterized.The chemo-sensing activity of L is detected by the naked eye for the aqueous carbonate anion in the pH range of 4 to 8. The selective 'turn-on' fluorescence occurs through the formation of a stable intermediate LâCO32-(1) following the PET mechanism. The limit of detection (LOD) is found 0.18 µM based on the absorbance-based assay.The quinonoid form of bromophenol unit binds strongly with CO32- through thiazole nitrogen and hydrazinic nitrogen. Further, the selective holding of CO32- anion over other planar tetranuclear anions (e.g., SO32-, NO3-) happens with several intra and intermolecular hydrogen bonds as envisaged by the DFT/TDFT study. The formation mechanism of LâCO32- is proposed based on experimental and theoretical studies. The biological experiments (MTT and cell imaging)reveal the non-cytotoxicity nature of L and the biocompatible uptake of L mostly in the cytoplasm at physiological pH.
Assuntos
Ânions , Carbonatos , Teoria da Densidade Funcional , Tiazóis , Cristalografia por Raios X , Tiazóis/química , Ânions/análise , Carbonatos/química , Humanos , Modelos Moleculares , Espectrometria de Fluorescência , Concentração de Íons de Hidrogênio , Limite de Detecção , Fenóis/química , Fenóis/análise , Corantes Fluorescentes/química , Corantes Fluorescentes/síntese químicaRESUMO
The perchlorate salt of (4-(4-methoxy phenyl)-2-(2-(1-pyridine-2-yl)ethylidene)hydrazinyl)thiazole (PytH·ClO4) and its metal perchlorate derivatives, namely, [Co(Pyt)2]ClO4 (1), [Mn(PytH)2](ClO4)2 (2), and [Ni(PytH)2](ClO4)2 (3), have been synthesized and characterized through single X-ray crystallography and spectroscopic methods. The ligand crystallizes in a space group P21/n in a nearly planar structure. The overall geometry of the complex salts is described as a distorted octahedron with a MN6 chromophore. The ligand (PytH·ClO4) behaves as a neutral N,N,N-tridentate donor toward the "soft" Mn(II) and Ni(II) centers, whereas the deprotonated ligand stabilizes the "hard" Co(III) center. The DNA binding constant (Kb) values of PytH·ClO4, 1, 2, and 3 are determined using the UV-vis spectroscopic method, and the Kb values are 9.29 × 105, 7.11 × 105, 8.71 × 105, and 7.82 × 105 mol-1, respectively, indicating the intercalative mode of interactions with CT-DNA. All the derivatives show effective antiproliferative activity against U-937 human monocytic tumor cells with IC50 values 4.374 ± 0.02, 5.583 ± 0.12, 0.3976 ± 0.05, and 11.63 ± 0.01 µM for PytH·ClO4, 1, 2, and 3, respectively. The best apoptosis mode of cell death is shown by 2 followed by PytH·ClO4 and 1 at an equivalent concentration of IC50 values. The combined molecular docking and dynamics simulation study evaluates the binding energies of anticancer agents, providing groove binding property with DNA. The 20 ns molecular dynamics simulation study reveals the maximum DNA binding stability of 2 corroborating the experimental results. The new class of metal derivatives of pyridine-thiazole can be used for advanced cancer therapeutics.